Primer

Part:BBa_K4812006:Design

Designed by: Rashik Chand   Group: iGEM23_NYU-Abu-Dhabi   (2023-10-08)


Scl2.28-1_F1_Forward


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 25
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The minimum overlap for Gibson Assembly was set to 25 nt.


Source

Primer Designed using NEB Assembly Tool. Contains part of genomic sequence of Scl2.28.

References